Synthetic oligonucleotides (oligos) are central to many cutting-edge biochemical applications across research and therapeutics. While solid-phase synthesis typically yields oligos with relatively high purity, they usually need further purification to avoid unintended side-reactions and interferences in downstream applications. Preparative HPLC is the preferred method for small to medium length oligos (10-60 bp). To increase productivity and maximize the profitability of your oligo research and development, finding the optimal LC purification platform is crucial.
In recent years, kratom has become an increasingly popular recreational drug around the world; consequently, this increase in popularity is matched by increasing concern over its potential to worsen the current opioid epidemic. As a result, further studies of kratom and its constituent compounds are necessary to better understand the mechanisms of action of these compounds and to evaluate possible therapeutic benefits of mitragynine or 7-hydroxymitragynine, present in kratom. Learn more in this application note.
Preparative chromatography, a method used to separate and purify target compounds from complex mixtures, plays a crucial role in the chemical and pharmaceutical industries. In either industry, preparative chromatography has roles in the early stages of drug development, pilot studies, and process development. In this article, you'll learn how we provide support throughout this process and enable our customers and partners to achieve their purification goals from the milligrams to the kilogram scale.
When choosing a high performance liquid chromatography (HPLC) system, it's important to consider how your future needs may evolve from your current needs. In this article, you’ll learn about the advantages of modular HPLC systems when it comes to current experimental needs and how the ability to swap out HPLC components safeguards the future of your system and your research.
Preparative high performance liquid chromatography (HPLC) is a well-established method for purifying target compounds. Method development is usually done at a smaller scale to define sufficient resolution between the peaks without wasting large amounts of sample. It is common to scale-up the purification from a smaller column to a larger preparative one - learn more in this technical note.
Liquid chromatography (LC) is the standard method used for purifying products in the discovery stages, as it is cost-effective, relatively simple to perform, and reliable. However, obtaining high purity and yield using this approach often comes at the cost of throughput, as purification runs must be lengthened to achieve greater separation between components. This article explores how users can enhance fraction collection efficiency and streamline the purification process by using the latest advances.
The Institute for Research and Innovation in Biomedicine (IRIB) focuses on developing innovative technologies for diagnosing, treating, and preventing diseases. In this case study, we explore how the researchers at IRIB are using preparative HPLC in their peptide purification drug discovery applications.
Preparative LC coupled with mass spectrometry (MS) is a well-established method for purifying target compounds. In this tech note, we demonstrate the use of a VERITY 271 LCMS system to perform mass-based purification of target peptides from a mixture. The system is controlled using TRILUTION® LC Software, which provides real-time and post-run MS and UV data.
Using the right systems can improve sample integrity in your sample separation workflows, helping labs to have greater confidence in their results. Here we outline what to look for in your sample separation instruments to remove risks of common threats to sample integrity.
Custom manufactured oligonucleotides require additional purification steps after synthesis to separate the full-length product from failing sequences and small impurities. In this infographic, we highlight how to accelerate purification for enhanced productivity and scalability with automation.