Gilson Connect
This application note outlines the automation of the Agencourt® AMPure® XP PCR purification system using Gilson’s PIPETMAX® 268. Designed to streamline PCR cleanup for next-generation sequencing workflows, the protocol uses SPRI magnetic beads to remove contaminants and isolate DNA fragments over 100 bp. The automated method improves reproducibility and throughput, processing up to 96 samples per run with a customizable magnetic bead separator. Applied to E. coli K12 gDNA libraries, the system produced high-quality fragments between 700–2000 bp with no contamination. The protocol is flexible, allowing users to adjust variables like reagent volumes, incubation times, and wash steps to fit diverse lab needs.
