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Technical Notes
Nitrosamines are a class of semi-volatile organic chemical compounds characterized by a nitroso group attached to an amine group. In this tech note we describe a method for automating US EPA Method 521 using the Gilson ASPEC® 274 Large Volume System
This technical note will explain different hardware configurations, as well as
software options, to use Gilson liquid handlers with TRILUTION® LH Software as autosamplers for spectrophotometric analysis.
This technical note shows how the use of a suitable Gilson liquid handler and TRILUTION® LH Software makes it is possible to automate post-purification analysis sample prep. Automating this process reduces manual handling errors
and provides traceability where previously there was none.
Centrifugal Partition Chromatography (CPC), also known as Counter Current Chromatography (CCC) is a preparative, pilot and industrial scale liquid purification technique that does not require traditional solid supports.
Gilson provides scientists with a range of manual and automated high-precision liquid handling tools to enable CRISPR/Cas9 gene-editing research. By choosing the right tool for the right job, convenience, productivity, accuracy, and precision can all be maximized to ensure reproducible and verifiable results.
Preparative LC coupled with mass spectrometry (MS) is a well-established method for purifying target compounds. In this tech note, we demonstrate the use of a VERITY 271 LCMS system to perform mass-based purification of target peptides from a mixture. The system is controlled using TRILUTION® LC Software, which provides real-time and post-run MS and UV data.
Preparative high performance liquid chromatography (HPLC) is a well-established method for purifying target compounds. Method development is usually done at a smaller scale to define sufficient resolution between the peaks without wasting large amounts of sample. It is common to scale-up the purification from a smaller column to a larger preparative one - learn more in this technical note.
In this technical note, the use of a PIPETMAX® automated liquid handling system is described for the pipetting of the plasma from the whole blood sample. PIPETMAX can be used to aspirate a precise volume of the plasma and transfer this to a suitable analysis or storage tube. If the specific laboratory workflow will benefit from it, then diluent can also be added to the analysis tubes, and the plasma can be mixed into the diluent as part of the same method.
The manual cyclization step in a peptide purification workflow is time-consuming and can easily become the rate-limiting step of the whole workflow, leading to delays in peptide production. Automating a drop-wise cyclization process using a liquid handler with a large rack capacity and incorporating automatic orbital shaking is an ideal solution to this workflow bottleneck. Learn more in this technical note.
Did you know that about 75% of known human disease genes have a recognizable match in the genome of the fruit fly? In this technical note, a lab uses the Gilson Fly Food Dispenser to dispense fly food into vials at regular intervals.